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Ovarian Steroids
Ovarian Steroids Androgens Stress Hormones Phytoestrogens

 

 

Estrogen and progestin production fluctuates over the ovarian cycle and pregnancy, providing a quantitative method for assessing ovarian cyclicity, ovulation and formation of a corpus luteum, and the progress of gestation. Fecal estradiol or estrone and progesterone or other progestins have proven to be reliable means of assessing ovarian function in primates and other mammals.

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The figure shows composite ovarian profiles using fecal estradiol and progesterone (bottom), serum estradiol and progesterone (middle) and sexual swelling ratings in three cycling sooty mangabey females.

Major Applications

Fertility
Reproductive seasonality and suppression
Occurence and timing of ovulation
Mating success
Conception and pregnancy loss

Capabilities

Fecal Steroid Extraction
Steroids are extracted from fecal samples using a solid phase method that provides a purified extract that minimizes contaminant interference with immunoassay. 
Fecal Estradiol
Estradiol is assayed using a highly specific [I125]E2 radioimmunoassay.
Fecal Progesterone
Progesterone is assayed using a highly specific [I125]P4 radioimmunoassay.

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